Assisted Reproductive Technology(ART)

Laser Assisted Hatching (Laser AHA)

The technique uses a laser to create an incision on the surface of the embryo, facilitating hatching and implantation
Laser assisted hatching basics An embryo is enclosed by a zona pellucidae composed of threee proteins (ZP1, ZP2, ZP3) for protection. Hatching occurs as the invisible shell becomes thinner from the expansion pressure of the embryo as it goes through cleavage. The hatched embryo forms the placenta through the implantation process from the signal transduction with the inner wall of the uterus. Embryos from IVF-ET may have abnormally thick shells due to various conditions in the body, leading to failure of implantation from hindered hatching process.

Candidates for Laser AHA

1. IVF-ET patients over the age of 35
2. Endometriosis patient
3. Recurrent failure cases of IVF-ET
4. Embryo with Thick ZP by Frozen-thawed
5. Embryo with unusually thick ZP

  • Technique used to thin or penetrate the ZP of the embryo using a laser with the wavelength of 0.48um
  • Safer compared to conventional methods(mechanical incision, chemical removal, proteolytic removal) and can minimize the exposure of the embryo
AHA(early Blastocyst)
Hatched Blastocyst